Globin mRNA reduction for whole-blood transcriptome sequencing

Globin mRNA reduction for whole-blood transcriptome sequencing

The transcriptome analysis of whole-blood RNA by sequencing holds promise for the identification and tracking of biomarkers; however, the high globin mRNA (gmRNA) content of erythrocytes hampers whole-blood and buffy coat analyses. We introduce a novel gmRNA locking assay (GlobinLock, GL) as a robust and simple gmRNA reduction tool to preserve RNA quality, save time and cost. GL consists of a p...

A guide to the whole transcriptome and mRNA Sequencing Service

Characterization of Whole Blood Gene Expression Profiles as a Sequel to Globin mRNA Reduction in Patients with Sickle Cell Disease

Global transcriptome analysis of whole blood RNA using microarrays has been proven to be challenging due to the high abundance of globin transcripts that constitute 70% of whole blood mRNA. This is a particular problem in patients with sickle cell disease, secondary to the high abundance of globin-expressing nucleated red blood cells and reticulocytes in the circulation. In order to accurately ...

Globin Reduction Protocol: A Method for Processing Whole Blood RNA Samples for Improved Array Results

Utilization of whole blood total RNA samples on GeneChip expression arrays has shown reduced detection sensitivity compared with using fractionated blood samples (e.g., peripheral blood mononuclear cells, or PBMC1). This Technical Note describes the development of a Globin Reduction Protocol (GRP) that can be used to specifically reduce the α1-, α2-, and βglobin mRNA population in whole blood t...

Whole transcriptome sequencing identifies tumor-specific mutations